Hybridomas Producing Goldfish Brain Specific Antibodies

摘要

New and powerful immunological methods were combined in a novel manner to detect minor goldfish brain specific antigens. (1) The method of differential tolerogenic selection was used to remove the antigenic competition from immunodominant common tissue antigens; (2) The hybridoma technology was used to construct cell lines which secrete monoclonal antibodies against goldfish brain specific antigen. This methodological approach can be applied to elucidate the molecular substrates underlying various normal and abnormal brain states.