Comparative Tissue Distribution of Cadmium in Mice Dosed with Partially Purified Extracts of Oyster

摘要

Two experiments were conducted to examine the relative tissue distribution of ingested cadmium (Cd) from edible oyster tissue. In the first experiment, oysters containing high or low concentrations of Cd were extracted in physiological buffer and the intact oyster, supernatant, or pellet were incorporated into diets at levels that would give equal Cd concentrations to each diet. Cadmium chloride was added to low Cd oyster diets to increase the Cd level to that found in the high Cd oyster diets. All diets were balanced for several constituents, including metals, and fed to mice for 7 days. Cadmium deposition in liver, kidney, duodenum, jejunumileum, and femur were similar for all treatments. Excretion of Cd was lower via the feces and higher via the urine in mice fed Cd occurring in the oyster pellet compared to mice fed intact oyster or oyster supernatant fractions. In the second experiment, the major soluble chemical form of Cd occurring in the oyster tissue was partially purified by a combination of centrifugation, gel permeation and ion exchange chromatography, and ultrafiltration techniques.