Teratologic Evaluation of Ethylene Chlorohydrin (CAS No. 107-07-3) in CD-1 Mice

摘要

Ethylene chlorohydrin (ECH), a widely used industrial chemical and reaction product of the gaseous sterilant ethylene oxide was evaluated for teratogenic potential in timed-pregnant CD-1 mice. ECH (0, 60 or 120 mg/kg/day, iv) in 5% dextrose was administered in a volume of 1 ml/kg of body weight on gestational days (gd) 4-6, 6-8, 8-10, or 10-12. All dams were weighed daily during treatment. No statistical significant expression of maternal toxicity was observed at the low dose level in any period of ECH administration. Evidence of embryotoxicity in the low-dose group was observed only following exposure to ECH on gd 8-10, a treatment which significantly decreased the average fetal body weight per litter. A significant dose related reduction in maternal weight gain was observed in the high-dose groups (120 mg/kg/day) during treatment for each of the four exposure periods. In addition, dams treated on gd 8-10 or 10-12 showed decreased gestational weight gain as well as decreased gravid uterine weight but no decrease in absolute maternal weight gain. ECH (120 mg/kg/day) produced dose related signs of embryotoxicity including a significant reduction in average fetal body weight per litter for each exposure period. Fetal toxicity was indicated by a significant increase in the percentage per litter of resorbed, non-live and affected fetuses. A significant increase in percent malformed fetuses per litter was noted in only the high dose group on gd 8-10.