Detection and Identification of 'Giardia' Cysts Using Immunofluorescence and Phase Contrast Microscopy

摘要

Detection and identification of Giardia cysts in water samples has been improved by the development of an immunofluorescent method that specifically stains Giardia cysts bright green and allows their easy detection against a black background. The report discusses aspects of the method--reagents and instrumentation--that must be considered and optimized by anyone wanting to adopt this technique for the detection of Giardia cysts.