Effects of Segregation and Selection on Instability of Plasmid pACYC184 in 'Escherichia coli'B

摘要

The authors use a mathematical model to analyze the dynamics of loss of nonconjugative pACYC184 from populations of Escherichia coli B in glucose-limited continuous culture. The model incorporates both plasmid segregation and selection against plasmid carriage. It is concluded that there is intense selection against plasmid carriage (s = 0.3 per culture generation), which amplifies the frequency of segregants arising de novo. (Copyright (c) 1987, American Society for Microbiology.)