Comparison of In vivo and In vitro Methods for Assessing the Effects of Bromobenzene on the Hepatic-Metabolizing Enzyme System

摘要

The effect of daily i.p. injections of 0, 0.05, 0.5 and 5.0 mmol/kg bromobenzene for 1 week on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay employing lindane ( -hexachlorocyclohexane) and by a battery of in vitro enzyme assays. The data in the study indicated that repeated pretreatment with bromobenzene for 1 week stimulated a dose-related increase in phase I reactions while inducing phase II reactions at the high dose (5 mmol/kg bromobenzene). The in vivo and in vitro assays showed good agreement. (Copyright (c) Toxicology Letters, Vol. 39, 1987.)