Raman and Surface Enhanced Raman Spectroscopy of Helix Destabilizing Proteins and Nucleotides

摘要

The thesis contains the results which have been obtained by application of Raman spectroscopy to biological molecules. Besides normal Raman spectroscopy (NRS) use is made of resonant Raman spectroscopy (NRS) and surface enhanced Raman spectroscopy (SERS). The interaction of proteins and nucleotides is essential for the regulation of molecular processes in living organisms. In chapter 2 an interpretation is presented of the Raman spectra of the helix destabilizing proteins gene product 32 (gp32) of bacteriophage T4 and gene product 5 (gp5) of bacteriophage M13 and complexes of these proteins with single stranded (poly) nucleotides. In chapter 3 an interpretation is presented of the SERS spectra of the base-molecules which occur in DNA. The bands in the spectra are assigned to normal coordinates. The orientation of the molecular plane of cytosine and thymine is probably tilted with respect to the surface. It is proposed for these molecules that carboxyl groups are positioned closest to the surface. Adenine adsorbs to the surface with the external aminogroup. The hypothesis is supported by the results of the investigation of methylated derivatives of adenine.