Comparison of Methods for the Estimation of the Thickness of Ultrathin Tissue Sections

摘要

For a number of quantitative electron microscopical techniques it is relevant to obtain an estimate of thickness t' of the section which, in general, will differ from the actual distance t between the two cuts that generate the section. To estimate t' of ultrathin sections, several techniques have been adopted in the past, both with or without the aid of the electron microscope and additional equipment, which are summarized in an addendum. In the present study five methods have been evaluated experimentally using sections of 10 different interference colors. (Copyright (c) TNO Medical Biological Laboratory 1987.)