DNA (Deoxyribonucleic Acid) Adducts of Nitropyrene Detected by Specific Antibodies

摘要

The production of specific antibodies to nitropyrenes is desirable for isolation and quantification of nitropyrenes in biological samples from exposed human populations. Dr. Groopman demonstrated the immunogenicity of chemically-modified proteins. He immunized rabbits with 1-nitrosopyrene-modified keyhole limpet hemocyanin that was prepared either by diazotization coupling of 1-aminopyrene to the protein, or by the nitroso condensation of 1-nitrosopyrene to DNA and the carrier protein. The nitropyrene protein antigen, when inoculated into rabbits, generated polyclonal antisera that reacted with 1-acetylaminopyrene, 1-acetylamino-6-nitropyrene, 1-acetylamino-8-nitropyrene, and the C-8-aminopyrene deoxyguanosine-DNA adduct, and 20- to 25-fold less strongly with 1-nitropyrene and 1-nitrosopyrene. The specificity of the antisera has not been proved, however. Unsuccessful attempts to produce specific antisera in mice and to generate monoclonals were also reported. (Copyright (c) 1987 by Health Effects Institute.)