Extraction and Purification of Microbial DNA from Sediments

摘要

A new method for the isolation of intracellular and extracellular DNA from a range of sediment types has been developed. The method is based upon the direct lysis of cells in the sediment, extraction of released DNA from the sediments and its subsequent purification by CsC1-EtBr gradient centrifugation and/or hydroxyapatite chromatography. Yields of 26 micrograms intracellular DNA and 1 microgram extracellular DNA have been obtained per gram of sediment. (Copyright (c) Elsevier Science Publishers B.V. (Biomedical Division).)