Volatile Organic Compounds in Whole Blood--Determination by Heated Dynamic Headspace Purge and Trap Isotope Dilution GC/MS

摘要

The method described was developed for the determination of a selected list of 30 organic compounds in whole human blood. The method is a heated dynamic head-space purge and trap gas chromatographic method using mass spectrometry in the limited mass scan mode. A whole blood sample is diluted with organic free water and the mixture fortified with isotopically labeled compounds. The mixture is subsequently heated to 50 deg C while stirred and the volatile components are purged from the mixture and collected on an adsorbent trap. The volatile compounds are thermally desorbed from the trap onto a wide-bore fused silica capillary column. Detection of the compounds is accomplished using mass spectrometry in the limited mass scan mode where only selected ions are scanned. Quantitation of the analytes is accomplished by using the specific ion responses from standards of the analytes and their corresponding labeled analogs or internal standards.