Cellulolytic and Xylanolytic Enzymes of 'Trichoderma reesei'

摘要

Enzymes hydrolyzing cellulose and xylan are important in the utilization of biomass in biotechnical processes. The work presented here concerns the cellulolytic and xylanolytic enzymes of Trichoderma reesei. Purification procedures, detection methods for the different components of the complex enzyme mixtures and some properties of the purified enzymes are described. T. reesei secretes into the culture liquid several enzymes which have very similar molecular and ionic properties. Beta-Glucosidase (1,4-beta-D-glucoside glucohydrolase), beta-xylosidase (1,4-beta-D-xylan xylohydrolase) and xylanases I and II (1,4-beta-D-xylan xylanohydrolase) could be separated from the culture filtrate on the basis of their failure to adsorb on amorphous cellulose and using gel- and ion-exchange chromatography. Cellobiohydrolases I and II (1,4-beta-D-glucan cello-biohydrolase) and endoglucanase I (1,4-(1.3;1,4)-D-glucan 4-glucanohydrolase) were purified by means of immunoaffinity chromatography. (Copyright (c) Valtion teknillinen tutkimuskeskus (VTT) 1988.)