Studies on the Metabolism and Biological Effects of Nitropyrene and Related Nitro-Polycyclic Aromatic Compounds in Diploid Human Fibroblasts

摘要

The cytotoxic effects of 1-nitropyrene (1-NP) and 1-nitrosopyrene (1-NOP) were compared in fibroblasts from normal persons, from excision-repair-deficient xeroderma pigmentosum (XP) patients, and from a patient with a predisposition to hereditary cutaneous malignant melanoma (HCMM). On the basis of concentration, 1-NOP was much more cytotoxic than 1-NP. When exposed to the same concentration of an agent, the normal and XP cells formed approximately the same number of initial DNA adducts, but the survival rate of the XP cells was much lower than that of the normal cells. On the basis of concentration, 1-NOP was much more mutagenic than 1-NP, and the XP cells were much more sensitive than normal cells to the induction of mutations by either compound, although the two compounds had virtually the same cell killing and number of initial adducts in a particular cell type. (Copyright (c) 1988 by Health Effects Institute.)