Standard Analytical Procedures of the NOAA (National Oceanic and Atmospheric Administration) National Analytical Facility, 1988: New HPLC (High-Performance Liquid Chromatography) Cleanup and Revised Extraction Procedures for Organic Contaminants

摘要

The Environmental Conservation (EC) Division of the Northwest and Alaska Fisheries Center conducts broad-ranging research into the nature and extent of pollution and its effects on marine and estuarine fish and their habitats. For 4 years the EC Division has participated in the NS&T Program, which monitors marine environmental quality at approximately 200 sites along the nation's coastlines. Hundreds of chemical analyses are needed annually by the NS&T Program to document the quality of the marine environment and assess trends. Wherever possible, standard methods are used to minimize the analytical variability among the participating laboratories. To expedite these analyses the EC Division's National Analytical Facility has developed a new instrumental method for the cleanup of sample extracts. The new method features the following: cleanup as effective as by former methods; better precision; less time required; capability of monitoring the chromatographic conditions; capability of being automated; and less highly pure solvent required. Through the Technical Memorandum, the new cleanup and revised extraction procedures are being made available to NS&T laboratories. The laboratory manual should also be useful to other federal, state, and local environmental programs which analyze for organic chemicals in sediments and organisms. These new procedures supersede Sections 7, 8, 10, and 11 of NOAA Technical Memorandum NMFS F/NWC-92 (MacLeod et al. 1985).