Insect Virus: Assays for Toxic Effects and Transformation Potential in Mammalian Cells

摘要

The increased sensitivity and reproducibility of two new assays developed for inclusion in Subdivision M of the Pesticide Assessment Guidelines are demonstrated. The IRS assay for toxicity and SHE/SA7 transformation assay were utilized together with presently recommended mammalian cell culture tests on a candidate viral pesticide. The results show that the IRS assay was capable of showing the cytotoxicity of the pesticidal agent in more different human cell lines and at lower dosages than the presently prescribed tests. The SHE/SA7 assay had significantly lower spontaneous transformation levels than the Balb/c 3T3 test. (Copyright (c) 1989, American Society for Microbiology.)