Application of Adenylate Energy Charge and Adenine Nucleotide Measurements as Indicators of Stress in 'Nephtys incisa' Treated with Dredged Material

摘要

Initial attempts at measuring AEC in Nephtys incisa yielded low values. A low energy charge most likely indicates a poor extraction of adenine nucleotides in tissue samples from individuals collected from a nonlimiting environment. Thus, there was a need to develop an extraction and handling procedure for N. incisa that consistently produced values for AEC ratios that were representative of in vivo values reported for actively metabolizing cells, (Atkinson 1977). The objective of the study was to evaluate the applicability of AEC as a measure of stress in N. incisa treated with dredged material under laboratory conditions and to determine the degree of variability inherent in the test.