Maintenance of Testosterone Production by Purified Adult Rat Leydig Cells for 3 Days In vitro

摘要

Using a preparation of highly purified, adult rat Leydig cells and conditions of culture which was found to optimize testosterone production during 24 h, attempts were made to maintain optimal testosterone production for 3 d. Leydig cells cultured on Cytodex 3 beads at 19% O2 in Dulbecco's modified Eagle's medium-Ham's nutrient mixture F12 (1:1; vol/vol) containing 0.5 mg/ml total bovine lipoproteins (