CCl4/CHCl3 Interaction Study in Isolated Hepatocytes: Selection of a Vehicle

摘要

Emulphor, ethanol and DMSO were evaluated as vehicles in studying the toxicity of CC14 and CHC13 in isolated hepatocytes. Appropriateness of the vehicle was determined by evaluating the following parameters: solubility of CC14 and CHC13 in the vehicle, cell injury (intracellular K+), cell death (LDH leakage) and lack of interaction (protection or enhanced toxicity) with CC14 and CHC13. The relative toxicity of the vehicles according to maximum no effect levels (v/v) was: emulphor (0.125%) > ethanol (1.0%) > DMSO (5.0%). Emulphor at toxic levels was inadequate to dissolve enough CC14 to evaluate in this system. Ethanol (5.0, 2.5, 1.0, 0.5%) was more toxic than DMSO and interacted with both CC14 and CHC13 to enhance toxicity of CC14 and CHC13; no interaction. These data suggest that DMSO should be the vehicle for evaluating the toxicity of CC14 and CHC13 and their mechanisms of action in the isolated hepatocyte. (Copyright (c) 1989 Society of Toxicology.)