Characterization of the H4IIE Rat Hepatoma Cell Bioassay as a Tool for AssessingToxic Potency of Planar Halogenated Hydrocarbons in Environmental Samples

摘要

An in vitro system, the H4IIE rat hepatoma cell bioassay, was characterized foruse in assessing the overall toxic potency of PCBs, PCDDs, and PCDFs in extracts from environmental samples. The in vitro bioassay of cytochrome P450IA1 catalytic activity in the H4IIE cells in response to planar halogenated hydrocarbons (PHHs) was repeatable over time and standards were reproducible among laboratories when dosing conditions were similar. Three common extraction/cleanup procedures tested had no adverse affect on the response of the cells and biogenic interferences were not encountered. The results of the bioassay can complement chemical residue analysis and direct the need for such analysis, as well as aid in the interpretation of biological effects data from environmental studies.