Treatment of Chlorophenol-Contaminated Water and Soils Using Immobilized Microorganisms

摘要

A Pentachlorophenol (PCP)-degrading Flavobacterium, a p-cresol-degrading Pseudomonas, and the lignin-degrading P. chrysosporium were all effectively immobilized in both alginate and polyurethane. The immobilized cells effectively degraded their target compounds, and the systems proved amenable to use in batch or fluidized bed reactors for degrading PCP or cresol contaminated water and soil. Polyurethane appears to be the immobilization matrix of choice for field application. Electron microscopy showed that cells were likely entrapped in small pores in the foam as opposed to being covalently linked to it. A field laboratory was assembled at a wood treating site and a 40 liter fluidized bed reactor containing foam-immobilized Flavobacterium was operated for a four week trial. The system achieved approximately 75% removal of PCP with a residence time of 0.5 hours, and approximately 90% removal with a residence time of 0.8 hours.