Production and Localization of Inulinases in 'Kluyveromyces' Yeasts

摘要

In the thesis a physiological study on the production and localization ofinulinase in Kluyveromyces yeasts, and especially in K. marxianus var. marxianus CBS 6556, is presented. It includes a biochemical study into the nature of the glycoprotein inulinase. As outlined above, the glycoprotein inulinase is in part associated with the cell wall of K. marxianus and partially released into the culture liquid. So far, no systematic studies on the relationship between growth conditions and glycoprotein localization have been performed. The experimental work was focused on answering two questions: Should inulinase be regarded as a special kind of invertase or as a different enzyme. Which factors influence the production and localization of inulinase.