Comparison of Extraction Methods for the Isolation of Lipids and PCBs from MusselHomogenate

摘要

A standard mussel homogenate was analyzed for total lipid mass, lipid classcomponents, and PCBs using four different extraction procedures in an attempt to determine the most appropriate method for use in bioaccumulation studies of lipophilic organic pollutants. The four procedures included: chloroform/methanol/water (similar to the classical Bligh and Dyer method), hexane/acetonitrile/water, and hexane/methanol/water extractions at controlled v/v/v ratios; and a hexane/acetonitrile method requiring minimal solvent volume ratio control. TLC analysis of the lipids, alone, was insufficient to determine qualitative distinctions between the extraction procedures studied; however, Iatroscan(TM) technology was able to provide information on both the qualitative and quantitative distribution of lipids in the various extracts.