Isolation and Characterization of Genes Involved in Basic Metabolism of theFilamentous Fungus 'Trichoderma reesei'

摘要

The filamentous fungus Trichoderma reesei is biotechnically interesting becauseof its excellent ability to secrete cellulases into its culture medium and as a potential production host for heterologous proteins. Although the cellulolytic properties of T. reesei have been thoroughly investigated, knowledge about the basic molecular biology of this fungus is still insufficient. To further develop T. reesei as a producer of cellulases as well as a host organism, it is necessary to understand the gene and promoter structure and the mechanisms of gene regulation in Trichoderma. For this purpose the glycolytic 3-phosphoglycerate kinase (pgk) gene was isolated from T. reesei by heterologous hybridization. In order to elucidate the relationship of T. reesei to other fungi, the ribosomal RNA repeat was isolated from T. reesei. The complete 5.8S rRNA gene and parts of the small and large rRNA subunit genes were sequenced. The sequence data from the rRNA and pgk genes was used to evaluate the relationship of T. reesei to other fungi.