Guidelines for Culturing the Japanese Medaka, 'Oryzias latipes'

摘要

The paper describes culture techniques for producing large numbers of all lifestages of Japanese medaka, Oryzias latipes, for use in biological research. The biology of the medaka is described as it relates to culturing practices and the physical systems used to maintain a large culture. The physical systems include water delivery apparatus, tanks, incubation pans, lighting, spawning substrates and other useful tools. The biological section addresses water temperature, spawning ratios, embryo incubation, juvenile rearing, and larval and adult feeding.