DNA Isolation from Small Tissue Samples Using Salt and Spermine

摘要

Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. These solvents pose some risk to the user and require special disposal procedures. The authors have previously reported a method for isolating DNA from peripheral blood lymphocytes by precipitation with spermine. This procedure was less effective with other tissues, especially lung, and the spermine precipitates often took several days to dissolve in buffer. The authors have modified their procedure to include a pre-extraction of proteins with concentrated NaCl, essentially as described by Miller et al. This current, improved procedure provides good recovery of purified DNA even with small samples (