Electrophoretic and Electro-Optical Studies on the Conformation and Susceptibility to Psoralen Crosslinking of Magnetically Oriented DNA

摘要

Gel electrophoresis and electro-optical birefringence measurements were performed on the replicative form of bacteriophage phi X-174 DNA subjected to orientation in a homogeneous stationary magnetic field. The conformation of this superhelical double-stranded form of DNA, and its sensitivity to intercalation and crosslinking by a psoralen derivative, were found to be unaffected by a 1 h exposure to a 2.15 Tesla field. In addition, no alteration was detected in the infectivity of the exposed phi X-174 DNA in E. coli bacterial hosts. (ERA citation 08:010793)