Enzymatic Determination of Oxygen in Aqueous Solutions. 1: Photosynthesis Rate in Cultivar of Cichorium Endivia L

摘要

A method based on the enzymatic stoichiometric reduction of oxygen and on the oxidation of ascorbic acid was applied to determine the quantity of oxygen in aqueous solutions. This reaction is oxygen-dependent and under anaerobic conditions the 2,6 dichloroindophenol oxidizes the excess ascorbic acid. The initial oxygen content of the solution is determined from the difference between the total ascorbic acid and the residual ascorbic acid. This method was used for titration of oxygen produced by leaves of Cichorium endivia l. immersed in carbonated water and exposed to sunlight. The results indicate that some strains of Cichorium exibit a higher photosynthetic rate than the others. Field tests should confirm whether the increased quantity of oxygen, produced by photosynthetic activity under the same conditions existing during the experimentation, corresponds to an increased net production of organic matter.