Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

摘要

Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.