Degradation of mix hydrocarbons by immobilized cells of mix culture using a trickle fluidized bed reactor. Final report: June 1992--June 1994

摘要

The microorganisms capable of degrading mix hydrocarbons were isolated from the soil samples collected from the hydrocarbon contaminated sites. The mix cultures were identified as Pseudomonas acidovorans, Flavobacterium indoltheticum and Phyllobacterium rubiaceum. The bacterial cells of mix cultures were immobilized in calcium-alginate solution in the form of beads. A trickle fluidized bed air-uplift-type reactor designed to study the degradation of mix hydrocarbons was filled with 0.85% normal saline containing the immobilized cells of mix culture. The immobilized beads were aerated with different amounts of CO(sub 2)-free air. The normal saline saturated with BTXs was circulated in the bioreactors at the rate of 2--4 ml/min. The biodegradation of BTXs by the immobilized beads of mix culture was monitored by determining the concentrations of the BTXs and the metabolites formed during their degradation in the samples at regular intervals using GC. The peaks obtained through the degradation of BTXs were not identified and quantified in this study.