Next-Generation Transcriptome Assembly.

摘要

Studying the dynamics and regulation of the transcriptome holds the key to understanding the function of a genome and the underlying biological processes. For a long time the accuracy and comprehensiveness of transcriptomics studies have been limited because our knowledge of the transcriptome has been partial and biased, since it is largely derived from gene prediction and limited EST evidence. Whole transcriptome sequencing by next-generation sequencing (NGS) technologies or RNA-Seq has started to reveal the complex landscape and dynamics of the transcriptome from yeast to human at an unprecedented level of sensitivity and accuracy.