Optimization and scale-up of fermentation process for production of microbial polysaccharide. Final technical progress report

摘要

This grant was awarded to provide for the scale-up of the process of production of a (1 (r arrow))-(beta)-D-glucan which is produced by Cellulomonas flavigena. One of the goals was to provide sufficient amounts of the polysaccharide polymer to conduct a field test of its usefulness in subterranean permeability modification procedures of enhanced oil recovery. During September and October, 1994, fermentations and recoveries were done by Abbott Laboratories, to develop a process to provide at least 400 lbs of the glucan polymer for field testing. Shake flask runs and four fermentation runs were completed. A summary of the fourth fermentation run, conducted in a 40,000 liter fermentor, follows.