Report of work performed by Dr. Ivancica Trosic in response to procurement request No. 4478H University of California, Los Alamos National Laboratory, Los Alamos, New Mexico

摘要

Rats were intratracheally instilled with polystyrene microspheres and/or asbestos particles (0.5 mg) suspended in PBS. Six animals from each group were dispatched n days 1, 7, 30, 80 and 150 days after their respective instillations. Their lungs were lavaged, and quantifications of lavaged fluorescent microspheres, analyses of distributions of microspheres in lung, phagocytes by type, quantification of unlavaged lung burdens of microspheres, and quantification of tracheobronchial lymph mode burdens of microspheres were performed. Data are presented herein describing lung retention kinetics of the microspheres, analyses of particle distributions in lung phagocytes over the course of alveolar phase clearlance, analyses of changes in the sizes of the lung's population of free cells.