Luminescent and electroactive labels for DNA sequencing and mapping

摘要

New labels for DNA based on metalloorganic compounds that are either electrochemically active or have long-lived luminescent excited states have been prepared. A derivative of the macrocyclic chelating agent, 1,4,7,10-tetracyclododecane-1,4,7,10-tetraacetic acid (DOTA) was used to attach the lanthanide (Ln(III)) ion to oligonucleotides. This ligand proved stable providing kinetically inert complexes with such metal ions.