Molecular genetic approach to understanding eukaryotic cellulose synthesis. Final technical report, March 15, 1995--March 14, 1997

摘要

In an attempt to understand eukaryotic cellulose synthesis, this research investigates the cellulose biosynthetic pathway of Dictyostelium discoideum. Attention is focused on the following: use of a newly developed method of tagged insertional mutagenesis to generate mutants in cellulose biosynthesis; and exploiting the monolayer culture system to identify differences in mRNA populations between DIF-induced (and cellulose-synthesizing) versus non-induced cells.