Final Harvest of Above-Ground Biomass and Allometric Analysis of the Aspen FACE Experiment.

摘要

Our objectives in year 3 were to (1) process fine root samples for DNA fingerprinting (2) complete the data analysis and publish results. During the 2009 field harvest, fine root samples were collected from 6 soil cores from each of 12 treatment rings. After sampling, the soil cores were cut horizontally into 10 cm slices. The fine roots from the 0--10 cm depth were placed on a 1 x 1 mm sieve and rinsed gently with water to separate soil and root material. The root material was placed in plastic bags moistened with water and stored at 4 C until the fresh tissue could be transported to the laboratory. Sample were sorted carefully using forceps and frequent washing to separate the individual fine root fragments. The fine roots of trembling aspen (Populus tremuloides Michx.) were separated from those of other plant species based on morphology and color.