Novel approaches to cancer targeting using epitope-binding properties that mimic monoclonal antibodies

摘要

The investigators have extensive experience in all of the techniques required for this project including: animal models of tumors, quantitative autoradiography, radiochemistry, peptide synthesis, organic synthesis, molecular biology, kinetic modeling and radionuclide imaging both with single photon and PET agents. Preliminary results and progress in the following areas are presented: (1) Establishment of an in vivo tumor model and successful targeting of this tumor using monoclonal antibodies raised to p185(sup erbB2), (2a) Screening of a synthetic peptide combinatorial library, (2b) Screening of a phage display peptide library, (3) Determination of the epitope recognized by ICR12, (4) Radiolabeling with (sup 99m)Tc, (sup 18)F and (sup *)I (radioiodines) , (5) High resolution positron emission tomography (PET) studies of tumors, and (6) Development of a high resolution ((approximately)mm) PET camera.