Effects of stress waves on cells

摘要

Laser induced stress waves are being used in a variety of medical applications,211u001eincluding drug delivery and targeted tissue disruption. Stress waves can also be 211u001ean undesirable side effect in laser procedures such as ophthalmology and 211u001eangioplasty. Thus, a study of the effects of stress waves on a cellular level is 211u001euseful. Thermoelastic stress waves were produced using a Q-switched frequency-211u001edoubled Nd:YAG laser (.=532nm) with a pulse duration of 4 ns. The laser radiation 211u001ewas delivered to an absorbing media. A thermoelastic stress wave was produced in 211u001ethe absorbing media and propagated into plated cells. The energy per pulse 211u001edelivered to a sample and the spot size were varied. Stress waves were 211u001equantified. We assayed for cell viability and damage using two methods. The laser 211u001eparameters within which cells maintain viability were investigated and thresholds 211u001efor cell damage were defined. A comparison of cell damage thresholds for 211u001edifferent cell lines was made.