Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report

摘要

During this fundion period, the laboraties of Drs. Anderson and Daunert have performed a considerable amount of work toward addressing the issues associated with small volume analysis necessary for single cell studies. In that respect, our research has been focused on (1) developing new assays that can be miniaturized and are suitable for small volume and single cell analysis; (2) fabricating pL-vials that simulate the volume of single cells and setting up instrumentation capable of low-volume detection; (3) developing reproducible and reliable microinjection techniques; (4) developing methods of anlaysis for biomolecules in the pL-vials and employing these assays in the detection of biomolecules in single cells. The accomplishments attained in all these areas are described below. A total of 24 publications and 35 presentations have resulted from this work.