Evaluation of Chloroethane Vg and Its Components in the Ames Salmonella/Mammalian Microsome Mutagenicity Assay with Attachments

摘要

The mutagenicity of Chlorothene VG solvent and its components (1,1,1-trichloroethane (93.33%), 2,-methyl-3-butyn-2-ol, nitromethane, nitroethane, t-amyl alcohol, and 1,2-butylene oxide) was evaluated in Salmonella tester strains TA98, TA100, TA1535, TA1537, and TA1538, both in the presence and absence of added metabolic activation by Aroclor-induced rat liver S9 fraction. Based on the results of preliminary bacterial toxicity testing, Chlorothene VG was tested at concentrations of 20,664, 41,328, or 165,310 ppm using the dessicator method. Chlorothene VG caused a reproducible positive response in tester strain TA1535, both in the presence and absence of S9 activation. The material did not cause a reproducible positive response in strains TA98, TA1537, or TA1538, either in the presence or absence of activation, although TA100 exhibited a trend toward a positive response.