Prevention of Sickling Human Erythrocytes in Vitro by Imidoester Treatment.

摘要

Previous studies have shown that sickling can be inhibited by increasing the hemoglobin-oxygen affinity. Evidence is presented here that brief treatment of human erythrocytes with a bifunctional cross-linking reagent dimethyl adipimidate at low concentrations prevents sickling,increases hemoglobin-oxygen affinity and preserves the physiological and physical properties of treated cells. Sickle erythrocytes treated with DMA washed and resuspended in original plasma showed the following properties under deoxygenated conditions:the number of sickle forms was reduced from 80to 4percent;the higher viscosity of sickle cells was brought into the range for normal cells;the K leak normally found under nitrogen was inhibited;red cells indices were shifted,for example,mean corpuscular hemoglobin 92to 97mu exp 3 ;osmotic fragility and auto hemolysis were decreased;glucose utilization and ATP synthesis were retained;the oxygen dissociation curve p50value was shifted from 30to 24mm Hg. Normal cells treated under similar conditions retain normal properties. Following these initial findings with dimethyl adipimidate three monofunctional and three other bifunctional imidoesters were also examined for their effect in inhibiting sickling in vitro.