Toward an Integrated BAC Library Resource for Genome Sequencing and Analysis. Progress Report (8/15/96-9/30/99)

摘要

BACs provide reliable access to specific pieces of genomic DNA that can easily be used for standard laboratory procedures including DNA sequencing and FISH mapping. BAC library resources will become much more valuable and efficient tools once the majority of the clones in the libraries are characterized and annotated. Several laboratories including TIGR, the genome center at University of Washington, and ourselves have been involved in determining the clone end sequences and restriction fingerprint patterns from each BAC in human library D and BAC libraries from other libraries. In the past three years, we have been focusing on a human BACEST project that aims to correlate unique ESTs or Unigenes to human BAC library D clones under the sponsorship of the DOE.