High-Throughput Microenvironment for Single-Cell Operations

摘要

This project was conducted as a feasibility study, in preparation for including this work in the forthcoming Instrumented Cell (IC) Strategic Initiative. The goal of the IC is to study individual cells; the goal of this feasibility study was to determine the best method for isolating large numbers of individual cells in a way that facilitates various types of environmental changes and intracellular measurements. We have the capability to do this with one cell, and sought to expand the number of cells that we could study simultaneously. Our specific goal for this feasibility study was to discover a way to isolate individual cells, and impale them on a nanopipette. This would enable samples to be introduced into and removed from a cell.