INCORPORATION OF FORMATE-C14 AND BICARBONATE-C14 INTO NORMAL AND X-IRRADIATED CHICK EMBRYOS

摘要

Embryonated eggs were subjected to 450 r x-irradiation on the 11th day of incubation. Promptly thereafter these and a comparable group of unirradiated eggs were inoculated with formate-C14 or bicarbonate-C14, and the incorporation of isotope into the RNA (ribonucleic acid) and DNA (desoxyribonucleic acid) bases was determined. In the embryos given formate-C14, the isotope content of the RNA purines in the irradiated embryos was either normal or, temporarily, elevated. The purines and thymine in the DNA of the irradiated embryos contained appreciably less isotope than the controls after a 2-day, as well as a 5-day incorporation period.