ULTRAVIOLET-INDUCED BIOCHEMICAL MUTANTS OF SACCHAROMYCES CEREVISIAE

摘要

Haploid yeast cells were exposed to ultraviolet and the survivors tested for nutritional requirements. A total of more than 5000 colonies was tested against twenty-three nutritional requirements, and a group of 138 mutants was obtained. These mutants have all been characterized with respect to their nutritional requirement, and many of them have been identified with particular genetic loci. Biosynthetic studies have beenperformed on some of the mutants. Three of the mutants were shown to be centromere-linked, and these were further shown to be linked with certain other known centro¬mere-linked genes.