ANALYSIS OF LOW-DENSITY LIPOPROTEINS BY PREPARATIVE ULTRACENTRIFUGATION AND REFRACTOMETRY

摘要

A simplified method for the analysis of both the glyceride-rich Sf20-105 and the cholesterol-rich Sf0-20 low-density lipoproteins is presented. It consists of serum lipoprotein fractionation by preparative ultracentrifugation and subsequent quantitative analysis by refractometry. Comparison of this technique with the technically more difficult analytic ultracentrifugal methodology reveals comparable results for these two principle low-density lipoprotein groups. One of the ad¬vantages of this proceedure is that it provides a reliable and reproducible means for quantitating the principal glyceride-bearing lipoprotein group - the Sf20- l05 lipoproteins.