Ultramicro Methods in Biochemistry. V. A Comparison of the Ultrmicro Determination of Phosphorus Using N-Phenyl-p-Phenylenediamine or 1-Amino-2- Naphthol-4-Sulfonic Acid. VI. The Determination of Serum Alkaline Phosphatase

摘要

An ultramicro method has been developed for the determination of serum inorganic phosphorus which uses 1 amino-2-naphthol-4-sulfonic acid as the reducing agent. This procedure is compared to an ultramicro method which applies the reaction induced by N-phenyl-p-phenylene-diamine. The acceptable wavelengths for the measurement of absorbance in both methods of analyses have been investigated and are discussed. The two methods are comparable in reproducibility and give results which justify the use of either one for the determination of phosphorus. The method using l-amino-2-naphthol-4-sulfonic acid has been applied to the determination of serum alkaline phosphatase with a substrate consisting of Beta-glycerophosphate in a barbiturate buffer at pM 9. 3. The ultramicro method is compared to the macro method from which it was derived. The comparisons of results with the two procedures are within acceptable limits to justify recommendation of the ultramicro method for the determination of alkaline phosphatase.