PHOTOREEAIR OF UV DAMAGE TO;DNA PURIFICATION AND PROPERTIES OF THE DNA PHOTOLYASE (the DNA-Photoreactivating Enzyme) January 2, 1971 – January 2, 1972

摘要

There is associated with the DNA-photolyase purified by affinity chroma-tography substances that have the spectral properties expected for the chromo-phore involved in DNA-photoreactivation. These substances can be diaiyzed in part away from the enzyme when it is concentrated in a collodion membrane. The fluorescence of the dialyzate is similar to that of the enzyme except for a blue-shift in the maxima of excitation and fluorescence emission. Two yellow green materials whose spectral properties mimicked those of known pteridines were separated from the dialyzate by paper chromatography. Heating the enzyme induces this blue-shift but addition of substrate, irradiated DNA, retards it.