Characterization of Mammalian Lymphocytes on the Basis of their Surface Receptor Sites for Specific Mitogens

摘要

The nature and specificity of the stimuli required for the continued proliferation of lymphocytes has been studied by DNA density transfer experiments in which restimulated ceils were incu¬bated in medium containing bromodeoxyuridine and also by double label autoradiography. The progeny of cells stimulated first by concanavalin A require an additional stimulus with concanavalin A to replicate. The majority of cells stimulated with phytohemagglutinin, streptolysin O, or staphylococcal filtrate must be restimulated with mitogen in order to replicate. We attribute the quantitative differ¬ence between concanavalin A and other mitogens in our experiments to the availability of the competitor, methyl-a-D-mannoside, which permits complete removal of concanavalin A. The progeny of cells stimulated by streptolysin C or staphylococcal filtrate can be restimulated by other mitogens, although in both cases slightly greater stimulation was obtained with the homologous mitogen.