METHOD FOR THE CONSTRUCTION OF THREE-DIMENSIONAL ELECTRON MICROGRAPHS

摘要

A method is described for the con¬struction of three-dimensional pictures from the computer output of the polytropic montage process for three-dimensional representation in electron microscropy. The final computer output is a series of two-dimensional images on photographic film, each of which corresponds to one increment in the third dimension. These film positives (dark images on light background) are stacked alternately with U-shaped spacer bars, with aluminum backing plates top and bottom. The entire assembly is bolted together firmly to form a mold. The mold is subsequently filled with a transparent plastic monomer and polymerized by heat.