RELATIONSHIP OF THE CHROMOSOME REPLICATION CYCLE TO THEnCELL DIVISION CYCLE OF BACILLUS SUBTILIS AND A PARTIALnCHARACTERIZATION OF A MEMBRANE-DEOXYRIBONUCLEIC ACID COMPLEX

摘要

The relationship of the chromosome replication cycle to the cell division cycle has been investigated in the bacterium Bacillus subtilis. In addition, a membrane-deoxyribonucleic acid complex has been isolated from this organism and partially characterized with respect to both its ONA and membrane components.nChromosome replication was studied in synchronously dividing cultures of B subtilis at three different generation times. At generation times of 38 and 58 minutes, initiation of a new round of replication was coincident with the termination of the preceding round, with both events occurring synchronously just prior to cell division. The time necessary to complete one round of replication was in both cases equivalent to the cell division time. At the third growth rate (126 minutes) chromosome replication did not occur synchronously.